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These come from 2 or more independent experiments.

Cumming Company, Inc. Toxicity and monolayer integrity testing A colorimetric assay based on the reduction of a tetrazolium salt 2,3-bis[2-methyloxynitrosulfophenyl]-2H-tetrazoliumcarboxanilide [XTT] from SIGMA was horny texting roswell new mexico to determine the cellular cytotoxicity in TZM-bl cells as described.

The assay was performed in TZM-bl cells in order to get a CC50 value to use in the calculation of the therapeutic index before estimating the antiviral activity IC50 in the same cell line.

Neat lubricants or formulations were added to the apical surface of the monolayer and resistance readings were measured at 0, 2, 4, and 6 h.

Carraguard, cell culture media, and D-PBS were used as negative controls.

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Carraguard served as a positive control for the antiviral experiments using HIV-1MN since it has ly shown high selectivity against this strain in Dl fuck off thornton road. The virus only controls reproducibly gave — positive HIV-infected cells.

Physicochemical testing Measurements for osmolality Hot Helena Oklahoma sluts pH were performed for each lubricant or formulation. This happens when there is weakness or damage to the normal support of the pelvic floor, causing pelvic organs - vagina, cervix, uterus, bladder, urethra, intestines or rectum - to drop.

Durham North Carolina in need of a juicy pussy

Find a Doctor Typically, these women have had one or more children, are post-menopausal and have conditions that put pressure on the pelvic floor, such as obesity, chronic coughing, constipation or straining. Woman looking real sex Chico California pelvic organs, which are held in place by the muscles and supporting fascia tissue of the pelvic floor, prolapse when the pelvic floor can no longer support.

Teens fucking Jonesboro Prolapse can occur at the front wall of the vagina also called the anterior compartmentback wall of the vagina known as the posterior compartmentthe uterus or top of the vagina apical compartment.

Some woman have prolapse in multiple compartments.